The 5-Second Trick For how HPLC works
The 5-Second Trick For how HPLC works
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These HPLC systems have two pumps with two unbiased solvent paths, two dosing units inside the autosampler, and two detectors, but preserve the footprint of just one HPLC system.
高效液相色谱仪工作原理 高效液相層析儀根據各種各樣的相互作用力來分離混合物。這種相互作用力通常是分析物及分析管柱之間的一種非共價性質。使用高效液相色谱时，液体待检测物在不同的時間被注入色谱柱，通过压力在固定相中移动，由于被测物中不同物质与固定相的相互作用不同，不同的物质顺序离开色谱柱，通过检测器得到不同的峰信号，每個峰頂都代表一個另外化合物的種類，最后通过分析比对这些信号来判断待測物所含有的物质。
In high-performance liquid chromatography (HPLC) we inject the sample, which is in Resolution type, into a liquid mobile stage. The cell stage carries the sample by way of a packed or capillary column that separates the sample’s components based mostly on their own ability to partition among the cell phase plus the stationary section.
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Cellular phase commences to move. The pump pushes the eluents or solvents from the system at a specified move fee.
The mobile period is buffer, plus the column packing comprises ionic groups. It really is employed to differentiate concerning anions and cations.
Consequently, owing to interactions Along with the stationary section, the constituent factors of a mixture migrate with the column at diverse speeds.
The information acquisition system documents and processes the indicators through the detector, allowing for that website generation of chromatograms along with the quantification of compounds.
In this particular specific instrument, Each individual pump sends its mobile stage to the mixing chamber in which they Incorporate to kind the ultimate cellular period. The relative pace of The 2 pumps decides the mobile period’s last composition.
For a typical rule, a two device transform during the polarity index corresponds to close to a ten-fold improve inside of a solute’s read more retention variable. Here is a simple case in point. If a solute’s retention variable, k
This may generate an Over-all equilibrium equation which dictates the quantity of the that could be connected with the stationary period and the amount of the that should be connected to the mobile section.
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One more technique for reducing a pulsed circulation is to incorporate a pulse damper in between the pump and also the column. A pulse damper is usually a chamber stuffed with an simply compressed fluid and a versatile diaphragm.
The sample injector introduces the sample into your HPLC system. Exact and exact sample injection is critical for obtaining trustworthy results.